Molecular Cloning: A Laboratory Manual
National Library of Australia. Search the catalogue for collection items held by the National Library of Australia. Sambrook, Joseph. Molecular cloning : a laboratory manual. Cold Spring Harbor, N. Users of the previous editions will recognize many of the organizational features in the experimental sections of this book. Nevertheless, the revision of the text has been extensive and detailed.
No other manual has been so popular, or so influential. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation.
7 editions of this work
Coverage includes techniques for isolating, analyzing and cloning both large and small DNA molecules; cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acids probes, mutagenesis, and DNA sequencing; and methods to screen expression libraries, express cloned genes in both pro- and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. In addition to annotations within protocols, information panels spread throughout the volumes provide insight into the reasons why methods are carried out in a certain manner and how techniques have evolved. The appendices are a compendium of reagents, vectors, media, techniques, suppliers, kits, and electronic resources. Book Info Updated text includes revised protocols and new material for students in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Describes techniques, cDNA cloning and more. Softcover, hardcover also available. Designed to be used in tandem with the Web site, molecularcloning.